01. Clarence Ashley - The Coo Coo Bird (The Cuckoo) [02:58]
02. Memphis Jug Band - On the Road Again [02:51]
03. Bukka White - The Panama Limited [03:12]
04. Hoyt Ming and His Pep Steppers - Indian War Whoop [03:11]
05. Frank Stokes - 'Tain't Nobody's Business If I Do, Pt. 1 [03:17]
06. Andrew & Jim Baxter - K. C. Railroad Blues [03:30]
07. Alfred G. Karnes - I Am Bound for the Promised Land [03:11]
08. Garfield Akers - Cottonfield Blues, Pt. 2 [03:21]
09. Bascom Lamar Lunsford - I Wish I Was a Mole In the Ground [03:20]
10. Bentley Boys - Down On Penny's Farm [02:48]
11. Whistler's Jug Band - Foldin' Bed [02:57]
12. Weems String Band - Greenback Dollar [03:10]
13. Mattie Delaney - Tallahatchie River Blues [02:49]
14. Cannon's Jug Stompers - Walk Right In [02:57]
15. The Carter Family - Bury Me Under the Weeping Willow [02:59]
16. Jim Jackson - Old Dog Blue [03:03]
17. Columbus Fruge - Bayou Teche [02:55]
18. Tommy Johnson - Cool Drink of Water Blues [03:34]
19. J. P. Nestor - Train On the Island [03:01]
20. Elder J.E. Burch - My Heart Keeps Singing [03:11]
21. Julius Daniels - Ninety-Nine Year Blues [03:06]
22. Earl Johnson and his Clodhoppers - I Get My Whiskey From Rockingham [03:03]
23. Rev. J. M. Gates - Death's Black Train Is Coming [03:13]
24. Jimmie Rodgers - Waiting for a Train [02:47]
25. Tenneva Ramblers - Darling, Where Have You Been So Long? [03:24]
26. Alabama Sacred Harp Singers - Rocky Road [02:46]
27. Amadie Breaux, Ophey Breaux & Cleoma Breaux - Ma Blond Est Partie [02:51]
28. Carolina Tar Heels - Peg and Awl [02:57]
29. Barbecue Bob - Chocolate to the Bone [02:54]
30. Eddie Head and Family - Down On Me [03:09]
31. Cleoma Breaux - Prenez Courage [02:57]
32. Dilly and his Dill Pickles - Pickin' Off Peanuts [03:29]
33. Nelstone's Hawaiians - Just Because [02:54]
34. Willie Walker - Dupree Blues [03:30]
35. Burnett & Rutherford - Ladies On the Steamboat [03:16]
36. Blind Willie McTell - Mamma, 'Tain't Long Fo' Day* [03:13]
37. Cuarteto Flores - Cecilia [03:12]
38. Los Borinqueños - La Coquetera [03:03]
39. Guty Cárdenas Y Lencho - Coconito [03:01]
40. Emmett Miller - Lovesick Blues [02:52]
41. Big Bill Broonzy - Long Tall Mama [02:51]
42. Two Poor Boys - John Henry Blues [02:49]
43. Lead Belly - Mr. Tom Hughes' Town [03:08]
44. Mississippi John Hurt - Louis Collins [03:01]
45. Blind Gary Davis - I Am The Light of the World [03:04]
46. Rev. F.W. McGee - Fifty Miles of Elbow Room [02:42]
47. Hopi Indian Chanters - Chant of the Eagle Dance [02:29]
48. Mike Hanapi & The Ilima Islanders - Hilo Hula [03:20]
49. Charlie Poole - If The River Was Whiskey [03:11]
50. Frank Hutchison - Stackalee [03:07]
51. Ernest Stoneman - The Wreck of the '97 [02:50]
52. Buell Kazee - Faded Coat of Blue [03:11]
53. Dock Boggs - Country Blues [03:02]
54. Uncle Dave Macon and His Fruit-Jar Drinkers - Sail Away Ladies [03:00]
55. Uncle Bunt Stephens - Sail Away Lady [03:00]
56. Eck Robertson - Sally Gooden * * * * * * * * * * * * * * * ** [03:10]
57. Don Richardson - Arkansas Traveller [03:07]
58. Lane Hardin - Hard Time Blues [03:20]
59. Charley Patton - Down the Dirt Road Blues [02:56]
60. Banjo Joe - Poor Boy, Long Ways From Home [03:11]
61. Blind Blake - Hastings Street [03:15]
62. Blind Lemon Jefferson - See That My Grave's Kept Clean [02:54]
63. Golden Melody Boys - Gonna Have 'Lasses In the Morning [02:43]
64. Memphis Minnie - New Orleans Stop Time [02:58]
65. Ma Rainey - Prove It On Me Blues [02:42]
66. Williamson Brothers & Curry - Gonne Die with My Hammer in My Hand [03:26]
67. Geeshie Wiley - Last Kind Words Blues [03:04]
68. Dixieland Jug Blowers - Banjoreno [03:15]
69. Beale Street Sheiks - It's a Good Thing [03:00]
70. Blind Willie Davis - Trust In God and Do the Right [02:49]
71. Sleepy John Estes - Someday Baby Blues [03:02]
72. Sam Collins - Lonesome Road Blues [03:06]
73. Willie Brown - Future Blues [02:57]
74. Henry Thomas - Bull Doze Blues [03:28]
75. Massey Family - Brown Skin Gal (Down the Lane) [02:47]
76. Dick Justice - Henry Lee aka Young Hunting [03:26]
77. William Moore - Old Country Rock [03:02]
78. Delma Lachney - La Danseuse [02:55]
79. Son House - My Black Mama, Pt. 1 & 2 [06:24]
80. Skip James - Cypress Grove Blues [03:16]
81. Robert Johnson - Cross Road Blues [02:32]
82. Lydia Mendoza - Mal Hombre [03:32]
83. Frenchy's String Band - Sunshine Special [03:11]
84. Robert Wilkins - Old Jim Canan's [02:54]
85. Amédé Ardoin - Les Blues De Voyage [02:57]
86. Stripling Brothers - The Lost Child [03:06]
87. Jaybird Coleman - I'm Gonna Cross the River Jordan Some of These Days [03:06]
88. Sol K. Bright - Tomi Tomi [02:43]
89. Mississippi Sheiks - Sittin' On Top of the World [03:16]
90. Richard Rabbit Brown - James Alley Blues [03:09]
91. Big Chief Henry's Indian String Band - The Indian Tom Tom [02:36]
92. Prince Albert Hunt's Texas Ramblers - Blues In a Bottle [03:25]
93. Bartmon Montet - Je Me Suis En Alle [02:53]
94. Blind Willie Johnson - Dark Was the Night, Cold Was the Ground [03:27]
95. Sol Hoopii & His Novelty Quartette - E Mama Ea [03:12]
96. Truett & George - Ghost Dance [03:02]
97. Roosevelt Graves & Brother - Woke Up This Morning (With My Mind On Jesus) [02:51]
98. Joseph Falcon - Allons A Lafayette [02:57]
99. Los Madrugadores - Corrido De Joaquin Murrieta, Pt. 1 & 2 [05:55]
100. Washington Phillips - Denomination Blues (Part 1 & 2) [05:39]